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Sorry, the application deadline for this position was 11/10/2019

Reference Number: 000001259
Posted Date: 11/5/2019
Closing Date: 11/10/2019

Department: Zane Cohen Centre
Position: Technician I

DEPARTMENT Zane Cohen Centre
EMPLOYMENT TYPE Temporary Full Time
HOURS OF WORK 37.5 hours per week
REPORTS TO Principal Investigator


The Lunenfeld-Tanenbaum Research Institute of Mount Sinai Hospital, a University of Toronto affiliated research centre, is one of the world's leading centres in biomedical research.  With ground-breaking discoveries in research areas such as diabetes, genetic disorders, cancer and women’s and infants’ health, the Institute is committed to excellence in health research and the training of young investigators.  Strong partnerships with the clinical programs of Mount Sinai Hospital ensure that scientific knowledge is used to promote human health.  Your significant contributions will assist in maintaining our momentum in advancing our research. 


An opportunity is available for a Technician I in the Clinical and Translational Research Unit (CTRU) at the Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital under the supervision of Dr. Ken Croitoru. The CTRU is an arm of the Host-Microbiome Research Network created to find the role of microorganisms in chronic disease development, with a focus initially on inflammatory bowel disease (IBD).


The Technician I plays an integral role in supporting the day-to-day operations of the lab; however, his/ her primary role is to provide technical support to the investigator, fellows and students.  He/ she may be required to work on one main project designed by the investigator.  Project(s) are generally outlined by the investigator or designate. 

Under the general supervision of the Principal Investigator and his delegates, the incumbent will be working on ongoing projects related to mucosal immunology and IBD research. The candidate will be responsible for carrying out in vitro and in vivo experiments with supervision by senior lab members or in partnership with other technicians as needed. The work will include sample processing from human studies i.e. working with blood, urine and stool; handling, aliquoting and storing samples with the aid of a database to catalogue samples and storage. The work may also include animal work (gavage, injections, breeding, health monitoring, and maintenance), as well as all procedures needed to obtain organs and tissues for cell isolation, culture, flow cytometry, and molecular biology.

The employee will be held responsible for the accuracy and reliability of results.  The employee will also be expected to record and demonstrate quality assurance, sample integrity and reliable sample identify. Employee will help others in the lab with ongoing work as needed and with general lab maintenance.


·         Under general supervision, performs routine standardized techniques, procedures and experiments (e.g. collecting, processing, storing and distributing various specimens)

·         The blood from humans or mice will be processed for serum, DNA extraction and isolation of fresh and viable peripheral blood mononuclear cells for future freezing, flow cytometry or in vitro functional analysis.

·         Stool samples will need to be processed and DNA extracted from bacteria samples from either mice or humans.

·         The applicant will be responsible for all activities related to receiving, shipping and processing samples from individuals involved in clinical studies.

·         The lab technician will work independently to perform semi-routine laboratory functions involving the utilization of standardized techniques and procedures. 

·         The employees will be held responsible for the accuracy and reliability of results.

·         Other duties include maintenance, electrophoresis, animal handling, recording and maintenance of records of experimental protocols and results with basic computation and plotting.

·         Monitors and maintains lab’s chemical/ cell stocks and/ or equipment, as well as order supplies

·         Assist other lab members


·         B.Sc. in Molecular, Cellular Biology or related field

·         1 year of laboratory experience


  • Demonstrated experience in isolating DNA from eukaryotic or prokaryotic organisms and in isolating and purifying WBC from blood samples.
  • Candidate must be comfortable and willing to work laboratory animals (mice; handling, dissection, tissue harvesting and processing) and in working with all human samples.
  • Must be able to exercise good judgment, exercise considerable independence and take initiative.
  • Demonstrated attention to detail and accuracy.
  • Experience with Molecular cloning techniques (DNA/ RNA isolation, ligation, digestion, amplification, purification, etc.)
  • Must be versatile, flexible and self-motivated
  • Must possess exceptional communication skills (verbal, written and presentation) and be comfortable engaging and exchanging information with other members of the lab
  • Demonstrated analytical and problem solving abilities with strong attention to detail
  • Experience with data management
  • Demonstrated knowledge of standard software
  • Must demonstrate satisfactory work performance and attendance record
  • Must be flexible with work hours (some days will start very early and/or end very late).

In accordance with Institute’s policy and legislated health and safety requirements, employment is conditional upon the verification of credentials, completion of a health review, and demonstrating proof of immunity and vaccination status of vaccine-preventable diseases.

The Lunenfeld-Tanenbaum Research Institute is a scent sensitive environment and all members of the community are expected to refrain from wearing or using scented products while visiting or working at the Institute. We also support a barrier-free workplace supported by the Institute’s accessibility plan, accommodation and disability management policies and procedures. Should you require accommodation at any point during the recruitment process, including accessible job postings, please contact the Lunenfeld-Tanenbaum Human Resources Department.


Posting open until November 10, 2019. We thank all candidates for applying. Only those selected for an interview will be contacted.

Hours: 37.5 hours per week

Contact Name: Online
Contact Email: Online
Contact Phone: Online
Contact Fax: Online


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