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CV Bank — Human Resources
Sorry, the application deadline for this position was 9/30/2020

Reference Number: 000001344
Posted Date: 7/20/2020
Closing Date: 9/30/2020

Department: LTRI
Position: Laboratory Technician

POSITION TITLE  Laboratory Technician
DEPARTMENT  LTRI
EMPLOYMENT TYPE  Temporary Part Time
HOURS OF WORK  7.5 up to 24 hours per week
EMPLOYEE GROUP  Non Union
REPORTS TO  Principal Investigator

The Lunenfeld-Tanenbaum Research Institute of Mount Sinai Hospital, a University of Toronto affiliated research centre, is one of the world's leading centres in biomedical research.  With ground-breaking discoveries in research areas such as diabetes, genetic disorders, cancer and women’s and infants’ health, the Institute is committed to excellence in health research and the training of young investigators.  Strong partnerships with the clinical programs of Mount Sinai Hospital ensure that scientific knowledge is used to promote human health.  Your significant contributions will assist in maintaining our momentum in advancing our research.

POSITION OVERVIEW:

An opportunity is available for a 1 year temporary part-time laboratory technician  at the Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital. Under the general supervision of the Principal Investigator and the Project Manager, the incumbent will be working on ongoing projects related to genetics, microbiome and IBD research. The candidate will be responsible for carrying out in vitro experiments with supervision by senior lab members or in partnership with other technicians as needed. The work will include sample processing from human studies i.e. working with blood, urine and stool; handling, aliquotting and storing samples with the aid of a database to catalogue samples and storage. The work may also include cell isolation, primary cell culture, flow cytometry, and molecular biology.

The employee will be held responsible for the accuracy and reliability of results.  The employee will also be expected to record and demonstrate quality assurance, sample integrity and reliable sample identify. Employee will help others in the lab with ongoing work as needed and with general lab maintenance.

DUTIES AND RESPONSIBILITIES:

  • The blood from humans will be processed for serum, DNA extraction and isolation of fresh and viable peripheral blood mononuclear cells for future freezing, flow cytometry or in vitro functional analysis.
  • Stool samples and intestinal tissue will need to be processed and bacterial DNA extracted from humans.
  • Extract RNA and miRNA from human blood, tissue and cell lines for transcriptomics
  • Assist senior research staff with grant writing and experiment budgeting
  • Tissue culture (primary cells, cell line propagation, cloning in expression vectors, transfections and stable cell line generation).
  • Immunohistochemical analysis in archived tissue sections and biochemical assays.
  • The applicant will be responsible for all activities related to receiving, shipping and processing samples from individuals involved in clinical studies.
  • The lab technician will work independently to perform semi-routine laboratory functions involving the utilization of standardized techniques and procedures. 
  • The employees will be held responsible for the accuracy and reliability of results.
  • Other duties include equipment maintenance, ordering,  lab cleaning,  electrophoresis, recording and maintenance of records of experimental protocols and results with basic computation and plotting.
  • Ensure equipment for clinical trials is properly maintained, logged and certified yearly
  • Ensure laboratory is in compliance with PHAC guidelines for Biohazard level 2 facilities and follows GcLPs  .

SKILLS/QUALIFICATIONS:

  • MSc in molecular biology or related field like immunology preferred or BSc in molecular biology or an acceptable equivalent combination of education and experience
  • 2-5 years of research laboratory experience
  • Demonstrated experience in isolating DNA from eukaryotic or prokaryotic organisms and in isolating and purifying WBC from blood samples.
  • Experience with primary cell culture and stem cells are particularly desirable.
  • Candidate must be comfortable and willing to work with human samples.
  • Excellent inter-personal and communication skills.
  • Must be able to exercise good judgment, exercise considerable independence and take initiative.
  • Demonstrated attention to detail and accuracy.
  • Must be flexible with work hours (some days will start very early and/or end very late).

In accordance with Institute’s policy and legislated health and safety requirements, employment is conditional upon the verification of credentials, completion of a health review, and demonstrating proof of immunity and vaccination status of vaccine-preventable diseases. Successful candidates will be required to provide two (2) written reference letters from their former employer(s)/supervisor(s).

The Lunenfeld-Tanenbaum Research Institute is a scent sensitive environment and all members of the community are expected to refrain from wearing or using scented products while visiting or working at the Institute. We also support a barrier-free workplace supported by the Institute’s accessibility plan, accommodation and disability management policies and procedures. Should you require accommodation at any point during the recruitment process, including accessible job postings, please contact the Lunenfeld-Tanenbaum Human Resources Department.

 

Posting open until September 30, 2020. We thank all candidates for applying. Only those selected for an interview will be contacted.

Hours: 7.5 up to 24 hours per week

Contact Name: Online
Contact Email: Online
Contact Phone: Online
Contact Fax: Online

 

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