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Sorry, the application deadline for this position was 9/23/2021

Reference Number: 000001524
Posted Date: 8/26/2021
Closing Date: 9/23/2021

Department: Lunenfeld Tanenbaum Research Institute
Position: Technician I

POSITION TITLE  Technician I
DEPARTMENT  LTRI - Jackson Lab
EMPLOYMENT TYPE  Regular Full Time
HOURS OF WORK  37.5 hours per week
EMPLOYEE GROUP  Non Union
REPORTS TO  Principal Investigator

The Lunenfeld-Tanenbaum Research Institute of Mount Sinai Hospital, a University of Toronto affiliated research centre, is one of the world's leading centres in biomedical research.  With ground-breaking discoveries in research areas such as diabetes, genetic disorders, cancer and women’s and infants’ health, the Institute is committed to excellence in health research and the training of young investigators.  Strong partnerships with the clinical programs of Mount Sinai Hospital ensure that scientific knowledge is used to promote human health.  Your significant contributions will assist in maintaining our momentum in advancing our research.

The Jackson lab is a newly established lab focused on the use of multiplexed imaging and single cell analyses to understand the formation and function of multi-cellular tissue subunits, communities of cells. Using imaging mass cytometry combined with multiple other technologies we track and investigate changing cellular interactions within pre-clinical models of disease and clinical patient samples. We are looking for a collaborative and meticulous research technician to work within a team setting to perform standard and multiplexed immunohistochemistry, validate antibody reagents for CyTOF applications, maintain our antibody database, and create high-dimension imaging mass cytometry data.

POSITION OVERVIEW:

The Research Technician will be responsible for contributing to and maintaining our imaging mass cytometry experimental pipeline from reagents to imaging equipment and will contribute to diverse collaborative academic and industry research projects.

DUTIES AND RESPONSIBILITIES:

  • Help manage tissue sample, antibody, cell line and plasmid inventories
  • Complete standardized immunostaining protocols
  • Test and validate antibodies for various immunostaining methods
  • Select and create control samples and tissues for reagent validation using molecular biology techniques
  • Tissue dissection, fixation, preparation, sectioning and processing
  • Acquire bright field, fluorescence and high dimension images using microscopy and our CyTOF Hyperion Imaging System
  • Record detailed consistent digital experimental notes and protocols
  • Coordinate usage and maintenance of shared lab equipment and reagents
  • Collaborate with other lab members to execute experimental work

SKILLS/QUALIFICATIONS:

  • Minimum Bachelor’s Degree in biological sciences. Additional work experience preferred.
  • Experience in the following techniques are an asset:
    • molecular biology (cloning, PCR, conjugation biochemistry)
    • mammalian cell culture (transfections, lentiviral infection, tissue culture)
    • immunohistochemistry and tissue processing (fixation, antigen retrieval, immunostaining, imaging)
    • microscopy imaging
    • statistics and data analysis using scripts and code
    • animal husbandry
  • Excellent interpersonal, communication, organizational, and record keeping skills
  • Ability and eagerness to learn new techniques
  • Ability to work independently and in a team environment

In accordance with Institute’s policy and legislated health and safety requirements, employment is conditional upon the verification of credentials, completion of a health review, and demonstrating proof of immunity and vaccination status of vaccine-preventable diseases. Successful candidates will be required to provide two (2) written reference letters from their former employer(s)/supervisor(s).

We are a fully committed to fairness and equity in employment and our recruitment and selection practices. We encourage applications from Indigenous peoples, people with disabilities, members of sexual minority groups, members of racialized groups, women and any others who may contribute to the further diversification of our Sinai Health community. Accommodation will be provided in all parts of the hiring process as required under our Access for People with Disabilities policy. Applicants need to make their requirements known in advance.

The Lunenfeld-Tanenbaum Research Institute is a scent sensitive environment, and all members of the community are expected to refrain from wearing or using scented products while visiting or working at the Institute. We also support a barrier-free workplace supported by the Institute’s accessibility plan, accommodation and disability management policies and procedures. Should you require accommodation at any point during the recruitment process, including accessible job postings, please contact the Lunenfeld-Tanenbaum Human Resources Department.

 

Posting open until September 23, 2021. We thank all candidates for applying. Only those selected for an interview will be contacted.

Hours: 37.5 hours per week

Contact Name: Online
Contact Email: Online
Contact Phone: Online
Contact Fax: Online

 

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